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It is extremely important to find out the bio-distribution of the drug before moving into the next phase of drug development. We are proudly offering the service to label your drug with either 125-I or 111-In. Radio-imaging and bio-distribution will be performed.

Material required
One mg of purified antibody, protein or small molecular drug. Tumor cell line of interest if it is not commercially available.

Phase I Isotope-labeling and SPECT radio-imaging
        • Establishment of tumors in 5 nude mice with tumor cells of interest
        • Labeling 0.2 mg antibody with 5 mci 125-I by the Iodogen method 
        • Purification of the isotope-labeled antibody using PD-10 column
        • Determination of the labeling efficiency, purification yield, purity and specific activity
        • Confirming the biological activity by ELISA against tumor cells or purified antigen
        • IV-injecting isotope-labeled antibody into two of the nude mice bearing tumors
        • Performing SPECT imaging at 4 different time points (4, 24, 48, and 72 hr for full length mAb)
        • Sending out the results to the customers by email

Phase II Isotope-labeling and Bio-distribution
       • Establishment of tumors in 25 nude mice with tumor cells of interest
       • Labeling 0.2 mg antibody with 5 mci 125-I by the Iodogen method
       • Purification of the isotope-labeled antibody using PD-10 column
       • Determination of the labeling efficiency, purification yield, purity and specific activity
       • Confirming the biological activity by ELISA against tumor cells or purified antigen
       • IV-injecting isotope-labeled antibody into 16 of the nude mice bearing tumors
       • Collecting 10 different organs plus blood from 4 of the mice at each time point. Total 4 time points (e.g. 4, 24, 48, 72 hr)
       • Reporting the data by email.

Phase III Isotope-labeling and Pharmacokenetics
       • Labeling 0.2 mg antibody with 5 mci 125-I by the Iodogen method
       • Purification of the isotope-labeled antibody using PD-10 column
       • Determination of the labeling efficiency, purification yield, purity and specific activity
       • Confirming the biological activity by ELISA against tumor cells or purified antigen
       • IV-injecting isotope-labeled antibody mixed unlabeled antibody at the given dosages into 5 Balb/c mice
       • Collecting blood from the mice at 8 various time points (e.g. 0.5, 1, 2, 4, 8, 24, 48, 72 hr)
       • Reporting the data by email.

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