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We will quickly clone out the variable regions of the monoclonal antibody from the hybridoma you sent to us.

Material required
Either one T-75 of live cells or a frozen vial of stock cells

Cloning of the variable regions of the mAb, Two Weeks

• Isolation of RNA from the hybridoma
• RT-PCR
• Cloning of the variable regions of the light chain and heavy chain using our proprietary primers by PCR
• Ligation of the PCR products into T-vector
• Sequencing 5 clones for each light chain and heavy chain
• Reporting the sequence data
 


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